Description
Intended Uses: The kit is an enzyme immunoassay for in vitro quantitative measurement of Anti-MMP9 in mouse serum, plasma and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with MMP9. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-MMP9 will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-MMP9 in the samples is then determined by comparing the O.D. of the samples to the standard curve